Real-Time Monitoring of Activated Bleomycin-Induced Cleavage of DNA with Piezoelectric Quartz Crystal Impedance Analysis

Abstract

A novel process analysis method, with a piezoelectric quartz crystal impedance technique based on the mass effect, for real-time monitoring the activated Bleomycin-induced cleavage of DNA process was proposed. The DNA-coated piezoelectric sensor was in contact with Bleomycin solution at the existence of H₂O₂ and Fe³⁺. The optimized experimental condition was determined through the experiments about the effect of pH and ionic strength. The experimental results indicated that activity of Bleomycin is maximal when pH = 8.0 and the ionic strength is low. Under the chosen experimental condition, Bleomycin concentration, which was in the range of 40–250 µg mL⁻¹, had an effect on the interaction, while Fe³⁺ concentration did not. And then the results about other antibiotics, Cefradine, Streptomycin, Benzylpenicillin, Doxycycline, Acetylspiramycin, showed that these antibiotics did not show the same behavior like Bleomycin.

Keywords:

• Bleomycin
• DNA cleavage
• Piezoelectric quartz crystal impedance

Acknowledgment

This work was supported by National Natural Science Foundation of China, Key Sci./Tech. Research Project and Doctorate Fund of Education Department of China.