A Study of the Interaction Between a New Reagent and Serum Albumin by Fluorescence Spectroscopy

Abstract

The synthesis of a new reagent, saturated fatty hydrocarbon substituting group compound N-n-hexyl-N ′-(sodium p-aminobenzenesulfonate) thiourea (HXPT), is described. The interactions between HXPT and bovine serum albumin or human serum albumin were studied by fluorescence spectroscopy. The binding constants of HXPT with BSA or HSA were determined at different temperatures under the optimum conditions. The binding sites were obtained and the acting force were suggested to be mainly hydrophobic. The effect of common ions on the binding constants was also investigated. A practical method was proposed for the determination of HXPT in bovine serum or human serum samples.

Keywords:

• N-n-hexyl-N ′-(sodium p-aminobenzenesulfonate)thiourea (HXPT)
• Bovine serum albumin (BSA)
• Human serum albumin (HSA)
• Fluorescence spectroscopy

Acknowledgments

This work was supported from the National Natural Science Foundation of China and partially financed from the Natural Science Foundation of Henan Province.