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Original Articles

Post‐chromatographic Detection of Progesterone Using Terbium‐Sensitized Luminescence

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Pages 131-142 | Received 01 Aug 2003, Accepted 01 Sep 2003, Published online: 16 Aug 2010
 

ABSTRACT

This report deals with a time‐resolved fluorometric determination of progesterone by reversed‐phase high‐performance liquid chromatography. The method was simple to use and performed on a C18 column, with acetonitrile/water 80:20 as mobile phase (flushed at 1 mL · min−1), and a 0.03 mol · L−1 Tb(NO3)3, 0.005 mol · L−1 SDS methanolic miscellaneous solution as post‐column reagent (added at 0.8 mL · min−1). The validation of the assay was performed for a 200‐µL injection, and it showed that this method presented no interferences with estrogens, and that it was linear in the range 5 × 10−6–75 × 10−6 mol · L−1 (correlation coefficient of 0.99), repeatable (3.7% intra‐day relative standard deviation (RSD) and 5.6% inter‐day RSD, at 40 × 10−6 mol · L−1), with a detection limit at 2.5 × 10−6 mol · L−1, and a quantification limit at 5 × 10−6 mol · L−1.

ACKNOWLEDGEMENT

The authors are grateful to Mrs. Pierre‐Eugène for technical assistance.

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