Accumulative Solid‐Phase Sorption of Pollutants as a Method for Hygienic and Environmental Inspection. III. Detection of Biowaste Using Boronylated Carrier

Abstract

Detection of various biological waste (BW) with the aid of complex formation on surface of composition “Biowadet” is described. This composition contains unmodified poly(methylmethacrylate) (PMMA) granules with situated on their surface phenylboronic acid. Its hydrophobic part is dipped into hydrophobic polymer matrix, while hydrophilic boronic groups are orientated into water media. These groups can bind two nearly situated hydroxyls that provides complex formation with various polyhydroxilic substances, for example with polysaccharides, lipopolysaccharides (LPS), glycoproteins, etc. These substances can be liberated by competition mechanism with glycerol in weakly acidic conditions. Periodate salts give oxidation of hydroxilic groups that gives a possibility to control experiment realization. “Biowadet”‐method may be applied to diverse purposes, such as an indication of reservoirs pollution, preparation of immunoglobulins (Ig), isolation of gram‐negative microbes (GNM) from water, etc. The simple method for environmental monitoring with application of blue dextran (BD) is proposed too.

Keywords:

• Hygienic and environmental inspection
• Biological waste detection
• Boronyl–polyhydroxils interaction
• Boronylated carriers
• Blue dextran as indicator
• Immunoglobulins investigation

Acknowledgment

A part of experiments was made in Institute of Biochemistry & Genetics, Ufa Scientific Center of Russian Academy of Sciences, Prospect Oktyabrya 69, Ufa‐city, 450054, Russia.