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Abstract
Determination of propham and chlorpropham in postharvest‐treated potato samples were examined using high‐performance liquid chromatography (HPLC) with chemiluminescence detection. After hydrolysis of the pesticides, aniline and 3‐chloroaniline metabolites were derivatized with dansyl chloride and the subsequent dansylated amines were injected into the chromatograph and detected by using an integrated chemiluminescence detection unit based on the bis(2,4,6‐trichlorophenyl) oxalate–hydrogen peroxide system. Conditions to obtain optimum derivatization and peroxyoxalate efficiencies were investigated. Dansylated metabolites were efficiently separated on a reversed‐phase C18 column eluted with 60% aqueous acetonitrile solution at 0.8 mL min−1 flow‐rate. The proposed method provided a very simple and sensitive procedure (limits of detection, 3.5 µg kg−1) to determine propham and chlorpropham in potato samples. The ensuing method is a useful choice for the determination of these pesticides as it provides limits of detection lower than those reported by existing chromatographic alternatives without the use of an additional pre‐concentration technique. Application was illustrated by the determination of these pesticides in potato samples after a treatment with a commercial formulation.
Acknowledgment
The authors gratefully acknowledge financial support from Spain's Dirección General de Investigación del Ministerio de Ciencia y Tecnología for the realisation of this work as a part of Project BQU2000–0905.
