Electrochemical Detection of Protein Using a Double Aptamer Sandwich

Abstract

We have developed an electrochemical sensor system for protein detection using two different antithrombin aptamers that recognize different parts of the target protein. In this case, thrombin was detected using a sandwich format comprised of a glucose dehydrogenase (GDH)‐labeled antithrombin aptamer and another aptamer that was immobilized onto the gold electrode for capturing the thrombin onto it. The electric current generated by the GDH when glucose was added was measured, and 1 µM of thrombin was detected selectively.

Keywords:

• Thrombin
• Aptamer
• Sandwich system
• Electrochemical detection