Chemiluminescence Microflow Injection Analysis System on a Chip for the Determination of Sulfite in Food

Abstract

Based on the chemiluminescence (CL) reaction between Ce (IV) and sulfite sensed by Rh6G and Tween 80 in acid medium, a new microflow injection analysis (µFIA) system on a chip for the determination of sulfite with chemiluminescence method was described in this paper. The channels of the µFIA system etched by CO₂ laser were 200 µm wide and 150 µm deep and the volume of reaction area was 1.80 µL. All reagents, including sample, were monitored by the injection pump with the rate of 50 µL/min. The linear range of sulfite concentration was 1.0–60 µg/mL with the detection limit of 0.5 µg/mL. The proposed method had good reproducibility with the relative standard deviation 3.0% (n = 7) for 20 µg/mL of sulfite and was very sensitive and simple. It had been successfully applied to the determination of sulfite in food.

Supported by a grant from the Science and Technique Ministry of China (2003BA310A05).

Keywords:

• Microflow injection analysis
• chemiluminescence
• sulfite

Notes

Supported by a grant from the Science and Technique Ministry of China (2003BA310A05).