Catalytic Kinetic Spectrophotometric Determination of Trace Amounts of Copper (II) in Biosamples using Tween–20 Microemulsion as Sensitizer

Abstract

A sensitive and selective kinetic catalytic spectrophotometric method has been developed for the determination of trace amounts of Cu (II) in Tween‐20 microemulsion medium. The method is based on the catalytic effect of Cu (II) on the reaction of Nuclear Fast Red with Potassium Periodate in the NH₃H₂O–NH₄Cl butter (pH 10.0). It is obvious that the microemulsion medium has higher sensitivity, better solubility, and stability than that of micelle and aqueous systems, so the new method is recommended for the determination of Cu (II). The reaction was followed spectrophotometrically at 530 nm. Calibration lines were in the range of 0.05–20.0 ng mL⁻¹, and the detection limit was 1.44 × 10⁻¹¹ g mL⁻¹. The optimum operating conditions and the corresponding kinetic parameters of the three systems were presented. The proposed method has been applied to many real samples such as urine and serum samples with satisfactory results. The recoveries were 94.8–106%, and the relative standard deviation (RSD) was below 4.6%.

Supported by the National Natural Science Foundation of China (No. 50373017), Natural Science Foundation (y2004B11), Science and Technology Program of Shandong Province (03C05) and Doctoral Startup Foundation of Jinan University (B0405).

Keywords:

• Microemulsion
• copper (II)
• kinetic catalytic method
• nuclear fast red
• spectrophotometry
• biosample

Notes

Supported by the National Natural Science Foundation of China (No. 50373017), Natural Science Foundation (y2004B11), Science and Technology Program of Shandong Province (03C05) and Doctoral Startup Foundation of Jinan University (B0405).

Additional information

Notes on contributors

Bin Du

Bin Du, a professor and doctoral supervisor, has been engaged in analytic chemistry and environmental science research. He acquired the degree of Doctor of Science from Chinese Academy of Sciences. He has been investigating the composition, property, and application of microemulsion.

Qin Wei

Qin Wei is a professor and DSc.