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Abstract
An indirect electrochemiluminescence (IECL) detection for capillary electrophoresis (CE) is presented in this article. IECL is based on the inhibition of Ru(bpy)3 2+/TPA system. The developed technique is successfully applied to the analysis of phenolic compounds: phenol, o‐chlorophenol, p‐nitrophenol, and 2,4,6‐trinitrophenol. The factors that influenced the separation and detection were investigated in detail. On the optimized condition, the tested phenols (phenol, o‐chlorophenol, p‐nitrophenol, and 2,4,6‐trinitropheno) could inhibit the ECL signal from the luminescence reagent, and the components of the mixture could be separated and recognized. In addition, when 2 mM phenol was the analyte, the relative standard deviations for migration time and negative peak height were 0.27% and 2.29%, respectively (n=6). And the linear correlation coefficient was 0.996 for phenol (0.5–5 mM). The preliminary results showed that the CE‐IECL might be applied to the detection of phenolic compounds or other samples.
Supported by the National Natural Science Foundation of China (No. 20299030 and 20335040), and National Key Basic Research Program 2001CB5102.
Notes
Supported by the National Natural Science Foundation of China (No. 20299030 and 20335040), and National Key Basic Research Program 2001CB5102.
