FLUOROALKYLATED POLYETHYLENE GLYCOL AS POTENTIAL SURFACTANT FOR PERFLUOROCARBON EMULSION

Abstract

So far, perfluorocarbon (PFC) emulsions have been manufactured based mainly on two surfactants, Pluronic F-68 and egg yolk phospholipids (EYP) for clinical use. However, they have been documented to induce inflammatory or allergic responses when PFC emulsions were injected into human bloodstream. The cause of these side effects is associated with the phagocytosis of emulsified PFC microparticles by cells such as macrophages. In order to lessen the side effects, it is logic to develop surfactants, which are more phagocytosis-resistant and biocompatible. In this study, a perfluoroalkylated polyethylene glycol (R_F-PEG) surfactant was synthesized by reacting perfluorooctanoyl chloride (C₇F₁₅COCl) with PEG of molecular weigh 8000. Both R_F-PEG 8000 and EYP were used to make PFC emulsions separately by an ultrasonic homogenizer. Individual PFC emulsions were then incubated with mouse macrophage J774A.1 cells to examine the degree of phagocytosis. From microscopic observation of cell morphology, our results showed that the process of phagocytosis was retarded to a large extend using the R_F-PEG surfactant. We also harnessed ¹⁹F-NMR to quantitatively detect the amount of PFC emulsions phagocytosed by J774A.1 cells. ¹⁹F-NMR result was consistent with the qualitative microscopic observation aforementioned.