Arginine Selective Biosensor Based on Arginase‐Urease Immobilized in Gelatin

Abstract

Arginase and urease enzymes were immobilized on the surface of pH electrode by using gelatin membrane which is then cross‐linked with glutaraldehyde. Sensor response was maximum when 2.5 mM, Tris‐HCl buffer (pH 8.5) was used at 25°C. The biosensor response depends linearly on arginine concentration between 0.025–0.310 mM with response time 10 min. Furthermore, application of the system for the arginine detection in serum samples was also tested.