Dexamethasone Influences Human Clock Gene Expression in Bronchial Epithelium and Peripheral Blood Mononuclear Cells in vitro

Naoto Burioka Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, JapanCorrespondence[email protected]

Miyako Takata Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan

Yoko Okano Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan

Shigehiro Ohdo Department of Clinical Pharmacokinetics, Division of Pharmaceutical Sciences, Graduate School, Kyushu University, Higashi‐Ku, Fukuoka, Japan

Yasushi Fukuoka Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan

Masanori Miyata Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan

Hiroshi Takane Department of Pharmacology, Faculty of Medicine, Tottori University, Yonago, Japan

Masahiro Endo Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan

Hisashi Suyama Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan

Eiji Shimizu Division of Medical Oncology and Molecular Respirology, Faculty of Medicine, Tottori University, Yonago, Japan

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Abstract

We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS‐2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼07:00 to 23:00 h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time‐PCR analysis. DEX stimulation of human BEAS‐2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.

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Dexamethasone Influences Human Clock Gene Expression in Bronchial Epithelium and Peripheral Blood Mononuclear Cells in vitro

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Dexamethasone Influences Human Clock Gene Expression in Bronchial Epithelium and Peripheral Blood Mononuclear Cells in vitro

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