Abstract
This study investigated the enhancedantitumor activity of Ad5-p53 in combinationwith mitomycin C (MMC) or cisplatin (DDP) in cervical cancer cell lines SiHaand C-33A. MMC and DDP inhibited the growth of SiHa and C-33A cells in a dose-dependentmanner, and the combination of MMC or DDP with Ad5-p53showed a stronger growth inhibition than those treated witheither Ad5-p53, MMC, or DDP alone. As evidencedby the formation of the ∼200 bp DNA ladder and the appearance of sub-G1peak, both MMC and DDP induced apoptosis in cervical cancer cells. Westernblot analysis of p53 showed that MMC/DDP did not induce the increase of p53protein in SiHa cells nor the increase of the cellular and nuclear p53 proteinin Ad5-p53 transfected Saos-2 cells. Takentogether, these results suggested that the combination of Ad5-p53and MMC/DDP may serve as an effective therapeutic regime forhuman cervical cancer treatment.