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Abstract
Methods were developed for the extraction and separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography (RP-ICP). Nucleotides (ATP, ADP, and AMP) were separated using a mobile phase of 15 mM TBAHS, 15 mM KH2PO4, and 7% acetonitrile. Inositol hexakisphosphate was separated using a mobile phase composition of 0.05 M formic acid:methanol (49:51 v/v) and 1.5 mL/100 mL of TBAOH. Extraction procedures were developed for the nucleotides which would be compatible with the RP-ICP system developed for their separation.