Abstract
The activity of SOD (superoxide dismutase) catalyzing dismutation of superoxide radical anion O2 − was studied by a modified NBT (nitroblue tetrazolium) method in aqueous solution, micellar solutions and lamellar liquid crystals (LLC) consisting of the surfactants CTAB and TX-100. The experiments showed that the inhibition by SOD of NBT reduction in organized assemblies of surfactants was weaker than in aqueous solution. The order of inhibition was as follows: in phosphate buffer (pH = 7.8) > in TX-100 micelle > in CTAB micelles > in TX-100 LLC > in CTAB LLC. The results were explained by decrease of α-helical content in SOD and difficulty in the transformation of SOD configurations in organized assemblies of surfactant, as well as by catalytic effects of micelles on NBT reduction.
ACKNOWLEDGMENT
The State Key Program Foundation (97021107) and National Natural Science Foundation (29733110) of China supported the work.