Stability of Alcohol Dehydrogenase (ADH) during a Suspended Droplet Drying and Storage

ABSTRACT

The retentions of alcohol dehydrogenase (ADH) activity during drying and storage were investigated at various temperatures and relative humidities for various carbohydrate solutions. The highest retention of ADH activity after drying was obtained for the mixture of trehalose and methyl-β-cyclodextrin. For a single dried droplet, the effects of humidity on the storage stability of ADH were reversed between the trehalose solution and the blend of trehalose and methyl-β-cyclodextrin. However, the addition of methyl-β-cyclodextrin decreased the residual activity of ADH during storage. The carbohydrate matrix structure after drying might influence mainly the storage stability of ADH encapsulated in the mixed carbohydrate of trehalose and methyl-β-cyclodextrin.

Keywords:

• Spray drying
• Drying of droplet
• Enzyme stability
• Crystallinity