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Abstract
This study investigated the polyhydroxyalkanoates (PHA) characteristics of denitrifying phosphate accumulating organisms (DNPAO) and non-denitrifying phosphate accumulating organisms (non-DNPAO) via a series of anoxic batch experiments in which the sludge was taken from a hybrid AS-biofilm pilot plant named the TNCU-I process. The results showed that 40.7% and 59.3% of the PHA was utilized in the anoxic and aerobic zones, respectively. Meanwhile, 47% and 53% of the phosphate was taken up in the anoxic and aerobic zones. The phosphate uptake amount per unit of PHA consumption (γPO4/PHA) in the anoxic and aerobic zones of the TNCU-I pilot plant were 0.83 mg PO4-P/mg PHA and 0.64 mg PO4-P/mg PHA. Furthermore, the batch experiments showed that the γPO4/PHA of DNPAO and non-DNPAO was 1.12 and 1.22 mg PO4-P/mg PHA, respectively. The difference of γPO4/PHA for non-DNPAO between the pilot plant and batch experiments may be caused by insufficient phosphate and the extra PHA consumption in the aerobic zone.
ACKNOWLEDGMENT
The authors would like to thank the National Science Council of the Republic of China for financially supporting this research under Contract No. NSC 89-2211-E-008-010.