Decolorization and Degradation of Xanthene Dyes by a White Rot Fungus, Coriolus Versicolor

Abstract

The decolorization of six xanthene dyes (conc. 100 μM) by a white rot fungus, Coriolus versicolor (C. versicolor), was investigated in liquid culture. The decolorization of Fluorescein, 4-Aminofluorescein, and 5-Aminofluorescein by the fungus was 85.0, 95.0, and 91.9% after 14 days incubation, respectively. However, no decolorization of Rhodamine B, Rhodamine 123 hydrate, and Rhodamine 6G was observed. The first three dyes also were decolorized with cell-free extracts from C. versicolor. The decolorization activity was 10.2, 6.7, and 7.2 μM min⁻¹ mg⁻¹, respectively. Thin layer chromatography (TLC) analyses indicated that degradation of Fluorescein was occurring with the detection of three degradation products.

Keywords:

• Xanthene dyes
• Decolorization
• Coriolus versicolor