Identification of Campania Citrus Limon L. by Random Amplified Polymorphic DNA Markers

L. Mariniello Department of Food Science, University of Naples “Federico II,”, Parco Gussone, Portici, Naples, ItalyCorrespondence[email protected]

M. G. Sommella Department of Food Science, University of Naples “Federico II,”, Parco Gussone, Portici, Naples, Italy

A. Cozzolino Department of Food Science, University of Naples “Federico II,”, Parco Gussone, Portici, Naples, Italy

P. Di Pierro Department of Food Science, University of Naples “Federico II,”, Parco Gussone, Portici, Naples, Italy

D. Ercolini Department of Food Science, University of Naples “Federico II,”, Parco Gussone, Portici, Naples, Italy

R. Porta Department of Food Science, University of Naples “Federico II,”, Parco Gussone, Portici, Naples, Italy

Abstract

Genotypes of 10 lemon (Citrus limon L.) cultivars of the Campania region (Southern Italy) have been studied by using Random Amplified Polymorphic DNA (RAPD) markers with 44 arbitrary 10-mer primers. Some of the studied cultivars (Sorrento, Procida, Amalfi, and Gloria d’Amalfi) have been successfully distinguished by their band patterns using five out of the 44 selected primers, confirming that RAPD technology provides a useful tool to identify specific cultivars.

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Acknowledgments

This work was supported by Ministero dell'Università e della Ricerca Scientifica e Tecnologica with contribution of Fondo Europeo Sviluppo Regionale, Progetto “Biotecnologie Mediche ed Agroalimentari.” We are in debt to Prof. Carbone for providing Citrus limon L. cultivars.

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Identification of Campania Citrus Limon L. by Random Amplified Polymorphic DNA Markers

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Identification of Campania Citrus Limon L. by Random Amplified Polymorphic DNA Markers

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