COULD CAPILLARY ZONE ELECTROPHORESIS OF TRYPTIC PEPTIDES BE USED FOR THE CHARACTERIZATION OF HEMOGLOBIN VARIANTS?

Changying Lin Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium

Béatrice Gulbis Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium

Emile Delobbe Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium

Patrick Robberecht Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium

Frédéric Cotton Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium

Bernard Fontaine Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium

Françoise Vertongen Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles (ULB), Route de Lennik 808, Brussels, B1070, Belgium

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Abstract

The present article describes a simple and rapid new peptide mapping procedure that could be used to assist identification of rare hemoglobin variants in clinical laboratories. Four hemoglobin variants were taken as example, namely Hb D-Ouled Rabah, Hb Marseille, Hb G-Philadelphia, and Hb Ube-2, and isolated by electrophoresis at alkaline pH. The globin chains were aminoethylated and, after tryptic digestion, the peptides were separated by a capillary zone electrophoresis method. Highly reproducible migration times of the peptides were obtained with intra-assay and inter-assay coefficients of variation of less than 1 and 2%, respectively.

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COULD CAPILLARY ZONE ELECTROPHORESIS OF TRYPTIC PEPTIDES BE USED FOR THE CHARACTERIZATION OF HEMOGLOBIN VARIANTS?

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COULD CAPILLARY ZONE ELECTROPHORESIS OF TRYPTIC PEPTIDES BE USED FOR THE CHARACTERIZATION OF HEMOGLOBIN VARIANTS?

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