Production of Murine Monoclonal Antibody to Fetal Hemoglobin

Yegneshwar S. Iyer Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

Manisha Patwardhan Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

Vaishali Pujari Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

Gayatri Shinde Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

Paritosh Keertikar Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

Swati Kulkarni Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

Roshan B. Colah Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

Dr. Dipika Mohanty Institute of Immunohaematology, Indian Council of Medical Research (ICMR), 13th Floor, New Multistoreyed Building, King Edward Memorial Hospital Campus, Parel, Mumbai, 400 012, India

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Abstract

Fetal hemoglobin (Hb F) is a major hemoglobin (Hb) component at birth. Hb F levels are markedly elevated in a number of inherited and acquired disorders. Measurement of Hb F levels is usually carried out by alkali denaturation which is not very accurate for low and high values. An accurate estimation of Hb F, and also of F cells, is desired in many hematological disorders like sickle cell disease, in monitoring the efficacy of hydroxyurea (HU) therapy, to assess feto‐maternal hemorrhage (FMH) during pregnancy and in the postpartum period. We have raised a murine monoclonal antibody to human Hb F, that accurately measures the number of F cells by flow cytometry. The antibody was found to be potent and specific for F cells.

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Production of Murine Monoclonal Antibody to Fetal Hemoglobin

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Production of Murine Monoclonal Antibody to Fetal Hemoglobin

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