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Hemoglobin
international journal for hemoglobin research
Volume 28, 2004 - Issue 4
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Short Communication

Molecular Characterization and Diagnosis of Hb Crete [β129(H7)Ala→Pro]

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Pages 339-342 | Received 24 Jun 2004, Accepted 15 Jul 2004, Published online: 24 Aug 2009
 

Abstract

We report the molecular characterization of Hb Crete [β129(H7)Ala→Pro] in a female subject from the Greek island of Crete. DNA sequence analysis revealed a 1368 GCC→CCC base substitution in exon 3 of the β‐globin gene, leading to the Ala→Pro amino acid change at codon 129. Both the proband and her mother, who were found to be heterozygotes for Hb Crete, presented with mild microcytic anemia and normal Hb A2 levels and iron metabolism indices. This is the first description of an heterozygous Hb Crete case, and also the first report on the molecular basis of Hb Crete. Moreover, the proposed NlaVI restriction enzyme‐based detection of Hb Crete at the DNA level is a fast and accurate approach, useful for molecular diagnostics.

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