Removing Peroxide Impurities from Ether Improves the Quality of Globin Chains for Biosynthetic Studies

Abstract

The diagnosis of the different forms of thalassemia is one of the important applications of analysis of globin chains. These analyses are done by high performance liquid chromatography (HPLC) using a MONO-S cation exchange column and ether is used for washing the globin powder in the final step. The presence of peroxide impurities in ether could change the structure of the globin chains. In the chromatograms, these modified globins appear as separated peaks next to the unmodified globin peaks. In these cases, the α/β ratio exceed by artifact the correct value. Our study demonstrates that diagnostic centers should ensure that the ether they use is pure.

• Thalassemia (thal)
• Cation exchange
• Globin chains
• Ether
• Peroxides