A Laboratory Method for Purification of Major Cow's Milk Allergens

Z. Pourpak Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, IranCorrespondence[email protected]

A. Mostafaie Research Center of Medical Biology, Kermanshah University of Medical Sciences, Kermanshah, Iran

Z. Hasan School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran

G. A. Kardar Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran

M. Mahmoudi Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Food allergy is responsible for the most frequent allergic reactions in children under 1 year of age. Diagnostic tests such as skin test or specific IgE assay usually need highly purified preparations of allergenic proteins. The aim of the present study was to purify three main cow's milk proteins: casein, α‐lactalbumin, and β‐lactoglobulin as allergenic extracts for first time in Iran.

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A Laboratory Method for Purification of Major Cow's Milk Allergens

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A Laboratory Method for Purification of Major Cow's Milk Allergens

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