Infection of Human Dendritic Cells by Dengue Virus Activates and Primes T Cells Towards Th0‐Like Phenotype Producing Both Th1 and Th2 Cytokines

Abstract

Dengue viruses (DV) infection is an important public health issue all over the world. Although the pathogenesis remains unclear, the overwhelmingly triggered immune responses have been consistently observed. Recently, we and other researchers demonstrated that the natural hosts for DV are dendritic cells (DC), the primary sentinels of immune system. In light of the significance of T cells in dengue virus pathogenesis, here, we examine the possible consequences of DC‐T cell interaction that is supposed to be happening in lymphoid tissues after infection. We showed that DV‐infected DC induced the interacting T cells to proliferate, to produce interleukin‐2 as well as to express activation markers on cell surface. Compared to mock‐infected DC, the infection of DC by DV also induced T cells to produce interleukin‐4, interleukin‐10 and interferon‐gamma, a cytokine pattern suggesting Th0 phenotype. Such an effect was either totally abolished or greatly reduced when DV were pre‐inactivated with heat or ultraviolet before infection. In addition, we demonstrated that such a Th0 phenotype shift of T cells was affected neither by different dosages of viruses that infected DC nor by different durations of DC‐T cell interaction. Our results provide a basic support for clinical observations and may be of help in understanding the pathogenesis of DV infection.

• Dengue viruses
• Dendritic cells
• T cells
• T helper 1/T helper 2
• Cytokines