LIFE IN ZERO MAGNETIC FIELD. III. ACTIVITY OF ASPARTATE AMINOTRANSFERASE AND ALANINE AMINOTRANSFERASE DURING IN VITRO AGING OF HUMAN BLOOD

Abstract

We investigated the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the serum of human blood during in vitro aging in normal and zero magnetic field (ZMF) for up to 72 hr at room temperature. We found a 24–31% apparent decrease of the enzymes' activities in ZMF conditions compared to controls aging in the normal geomagnetic field. The presence of these enzymes in the serum is mainly due to hemolysis. However, hemolysis is stronger in ZMF conditions. Therefore, the amount of enzymes released into the serum is correspondingly higher in these conditions. This leads to the conclusion that AST and ALT activities diminished by ZMF to a much greater extent than the apparent effect. For example, a 72-hr aging leads to a minimum five times reduction in the enzymatic activity in the blood serum. The loss of activity could be explained by denaturation and degradation processes, which proceeded more rapidly in ZMF conditions.

• Aging
• Blood
• Cell membranes
• Enzymatic activity
• Hemolysis
• Magnetic field