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Abstract
Centrifugal partition chromatography linked with evaporative light scattering detection has been investigated to achieve the large scale-up isolation of oraposide which is a phenylpropanoid glycoside having a radical-scavenging activity. A two-phase solvent system has been studied to improve the yield of pure product and to decrease the cost of purification. A comparison with preparative HPLC has been made.
ACKNOWLEDGMENT
The authors would like to thank, especially, A. Saunois and S. Lhermite (Parfums Christian Dior) for improving the extraction method of the Orobanche rapum crude extract.
