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Original Articles

PURIFICATION OF PEG-PROTEIN CONJUGATES BY CENTRIFUGAL PRECIPITATION CHROMATOGRAPHY

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Pages 1973-1979 | Received 28 Dec 1999, Accepted 31 Jan 2000, Published online: 06 Feb 2007
 

Abstract

Preparative scale purification of PEG-protein conjugates by centrifugal precipitation chromatography is discussed. By utilizing the transport of ammonium sulfate across a membrane and the centrifugal force, an ammonium sulfate gradient is formed and used for differential precipitation of PEG-protein conjugates. Native proteins can be separated from PEG-protein conjugates by using this technique.

ACKNOWLEDGMENT

One of the authors, Terasut Sookkumnerd, is greatly indebted to Suranaree University of Technology, Thailand and Ministry of University Affairs, Royal Thai Government for providing financial support under the Faculty Development Program during his study at Lehigh University.

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