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Journal of Liquid Chromatography & Related Technologies Volume 23, 2000 - Issue 16
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Original Articles

SEPARATION OF UNDERIVATIZED SMALL PEPTIDES ON A POROUS GRAPHITIC CARBON COLUMN BY ION-PAIR CHROMATOGRAPHY AND EVAPORATIVE LIGHT SCATTERING DETECTION

A. Ait Adoubel Université d'Orléans, CNRS UPRES-A 6005, BP 6759, Orléans, Cedex 2, 45067, France
,
S. Guenu Antenne Scientifique Universitaire de Chartres (Université d'Orléans), 21 rue de Loigny la Bataille, Chartres, 28000, France
,
C. Elfakir Université d'Orléans, CNRS UPRES-A 6005, BP 6759, Orléans, Cedex 2, 45067, France
&
M. Dreux Université d'Orléans, CNRS UPRES-A 6005, BP 6759, Orléans, Cedex 2, 45067, France
Pages 2433-2446 | Received 24 Feb 2000, Accepted 12 Apr 2000, Published online: 16 Aug 2006
 
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Abstract

Separations of small peptides (di-, tri- and tetrapeptides) using ion-pair chromatography and evaporative light scattering detection have been investigated with a porous graphitic carbon stationary phase, Hypercarb®. Five perfluorinated carboxylic acids (trifluoroacetic (TFA), pentafluoropropanoic (PFPA), heptafluorobutanoic (HFBA), nonafluoropentanoic (NFPA) and tridecafluoroheptanoic (TDFHA) acids) have been evaluated as ion-pairing agents. Influence of the percentage of organic modifier, concentration, and chain length of the ion-pairing agent on the retention factors, k, has been examined. Equilibration times were also studied. Separations of dipeptides or tri- and tetrapeptides are presented using HFBA and NFPA at 20 mM and TDFHA at 2.5 mM.

ACKNOWLEDGMENTS

The authors gratefully acknowledge the financial support of the Conseil General of the Eure-et-Loir (France) and Parfums Christian Dior (Saint-Jean de Braye, France).

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