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Abstract
A reverse-phase high performance liquid chromatography (HPLC) method for the determination of glucosamine in nutritional supplements was developed and validated based on USP guidelines. Separations were performed using a MetaChem Inertsil ODS-3 column with an isocratic mobile phase consisting of 10% Methanol, 90% 0.005M octanesulfonate, pH = 2.1. Glucosamine was detected using a refractive index (RI) detector. The octanesulfonate ion pairing agent allowed for retention on a C-18 column.
The method proved highly reliable with respect to standard performance characteristics. The method allows for convenient quality control in the growing nutritional supplement market.
ACKNOWLEDGMENTS
This work was supported by Nutramax Laboratories®, Incorporated. Furthermore, we thank Jeff Niemeyer for his insightful discussions.