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Abstract
The quantitation of 2,6-pyridinedicarboxylic acids (2,6-PDA) in fruit juice can be achieved rapidly utilizing High Pressure Liquid Chromatography (HPLC).
The best chromatographic performance was obtained with an aqueous mobile phase containing 15 mM tetrabutylammonium phosphate and 2 mM EDTA; the pH was maintained at 7.2 by 10 mM phosphate buffer.
The influence of the eluent parameters on retention of PDAs has been investigated in order to elucidate the separation mechanisms involved in the ion pair chromatography of these ionizable substances.