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Abstract
Purification of PEG-protein conjugates by countercurrent distribution (CCD) in aqueous two-phase systems is reported. By using CCD in 19.36% PEG 3400/14.68% potassium phosphate, we can separate PEG-lysozyme conjugates, which contains an average number of 1.14 PEG per lysozyme, into three peaks with 55 number of transfers. Further analysis of the samples reveals that these three peaks mainly represent unmodified lysozyme, (PEG)1-lysozyme and (PEG)2-lysozyme.
ACKNOWLEDGMENT
One of the authors, Terasut Sookkumnerd, is greatly grateful to Suranaree University of Technology, Thailand and Ministry of University Affairs, Royal Thai Government for providing financial support under the Faculty Development Program during his study at Lehigh University.
