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Journal of Liquid Chromatography & Related Technologies Volume 23, 2000 - Issue 4
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Original Articles

SIMULTANEOUS DETERMINATION OF CATHARANTHUS ALKALOIDS USING REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

D. V. Singh Central Institute of Medicinal and Aromatic Plants , Lucknow, 226015, India
,
A. Maithy Central Institute of Medicinal and Aromatic Plants , Lucknow, 226015, India
,
R. K. Verma Central Institute of Medicinal and Aromatic Plants , Lucknow, 226015, India
,
M. M. Gupta Central Institute of Medicinal and Aromatic Plants , Lucknow, 226015, India
&
Sushil Kumar Central Institute of Medicinal and Aromatic Plants , Lucknow, 226015, India
Pages 601-607 | Received 03 Mar 1999, Accepted 03 Apr 1999, Published online: 06 Feb 2007
 
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Abstract

A rapid and simple reverse phase liquid chromatographic method has been developed for the simultaneous quantitation of anticancerous drugs vincristine, vinblastine and their precursors catharanthine and vindoline. Peak purity evaluation of these compounds in plant extract has also been studied using photo–diode array–UV detector.

ACKNOWLEDGEMENT

The authors are indebted to Prof. P. Potier (National Centre for Scientific Research, Yvette, Cedex, France), for the generous gift of vindoline and catharanthine standards.

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