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Abstract
A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycations (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method,examined in various matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its versatility, simplicity, and short time of determination of polyamines and related substances of importance in prokaryotes and eukaryotes, even in different matrixes, using a standard HPLC equipment (UV detection at 254 nm), with good resolution, sensitivity, and reproducibility.
ACKNOWLEDGMENTS
This work was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT; BID 802 OC AR PICT 0043) and Universidad de Buenos Aires (UBA), Argentina. The authors thank laboratories of Dr. R. S. Calandra and of Dr. J. Tezón for providing seminal plasma and Dr. J. C. Calvo for critical reading of the manuscript.