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Original Articles

DETERMINATION OF FUROSINE IN HYDROLYZATE OF PROCESSED MILK BY HPLC USING A NARROW BORE COLUMN AND DIODE-ARRAY DETECTOR

, , , &
Pages 717-726 | Received 03 May 1999, Accepted 19 Jul 1999, Published online: 17 Aug 2006
 

Abstract

A simple, rapid, sensitive, reproducible, and accurate HPLC procedure employing a narrow bore reversed phase column and a diode-array detector is proposed for the determination of ϵ-N-2-furoylmethyl-L-lysine (furosine) in acid hydrolyzed of milk and powdered milk samples.

After optimization of the variables involved, the method was characterized and validated in terms of selectivity, repeatability, sensitivity, and accuracy and applied to determine furosine content in hydrolyzed milk samples.

ACKNOWLEDGMENTS

We are grateful to Dr. G. Attioli (SAGIT, Division of Unilever Italia S.p.A., Cisterna di Latina, Italy) for the kind gift of samples of dried milks and dried milk whey. E. C. was the recipient of a postdoctoral fellowship from C.N.R. (National Research Council).

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