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Abstract
The separation of oxybutynin enantiomers (racemic mixture of two enantiomers) was studied on an ovomucoid column. The optimized chiral separation baseline resolved the enantiomers in less than 10 minutes. Chromatographic variables that were found to have an effect on the chiral separation were studied and include: mobile phase pH, mobile phase ionic strength, type and concentration of organic modifier added to the mobile phase, column temperature, and the amount of analyte injected.
The enantiomeric separation was optimized on the ovomucoid column based on how each variable affected the separation. Calibration curves for a standard were linear over a range of 8.36 to 668.8 μg/g (ppm) with a correlation coefficient of 0.999 for both enantiomers. A detection limit of 4.5 μg/g and a quantitation limit of 9.0 μg/g were also found.