USE OF COUNTERCURRENT CHROMATOGRAPHY (CCC) TO SEPARATE MIXTURES OF ARTEMISININ, ARTEMISITENE, AND ARTEANNUIN B

Abstract

A series of solvent systems was developed for CCC to separate mixtures containing variable amounts of artemisinin, artemi-sitene, and arteannuin B. To purify multigram quantities of artemisinin by CCC it was necessary to maximize the amount of a mixture that could be dissolved in a fixed quantity of the solvent mixture.

ACKNOWLEDGMENTS

The authors thank Col. John Scovill at the Walter Reed Army Institute of Research, Washington D.C. for the mixtures of artemisinin, artemisitene and arteannuin B employed in this study. E. W. was a participant in the Colgate/NIH Study Group during the summer of 1998.