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Original Articles

RAPID ASSAY FOR THE DETERMINATION OF ZINC BACITRACIN IN FEED BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

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Pages 1083-1088 | Received 03 Sep 1999, Accepted 14 Sep 1999, Published online: 06 Feb 2007
 

Abstract

A simple method for the determination of zinc bacitracin in feed is presented. The samples were extracted with methanol-hydrochloric acid and 1 mL of the supernatant was evaporated to c. 50 μL. After adding water and mixing, the water layer was cleaned up using LMS solid phase extraction columns, and injected to the liquid chromatography-atmospheric pressure ionization ion spray. The lower limit of quantification was 4 mg/kg in chicken and swine feed.

ACKNOWLEDGMENT

We are grateful to the Norwegian Research Council for financial support.

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