SEPARATION AND IDENTIFICATION OF TOFISOPAM STEREOISOMERS BY HYPHENATED HPLC-CD TECHNIQUE

F. Zsila Institute of Pharmaceutical Chemistry, Semmelweis University of Medicine, Hõgyes Endre u. 9, Budapest, H-1092, Hungary

A. Gergely Institute of Pharmaceutical Chemistry, Semmelweis University of Medicine, Hõgyes Endre u. 9, Budapest, H-1092, Hungary

P. Horváth Institute of Pharmaceutical Chemistry, Semmelweis University of Medicine, Hõgyes Endre u. 9, Budapest, H-1092, Hungary

Gy. Szász Institute of Pharmaceutical Chemistry, Semmelweis University of Medicine, Hõgyes Endre u. 9, Budapest, H-1092, Hungary

Abstract

The published method allows the separation of the four isomers of the anxiolytic 2,3-diazepine derivative, tofisopam. Assignment for the peaks of the two central and two helical isomers is performed by using the chromatogram of the pure tofisopam enantiomers and also considering the conformer-equilibrium was suggested by previous authors. The detection has been made by CD. The in situ CD spectra of the peaks, obtained by stop-flow method, definitely show different chiral character.

ACKNOWLEDGMENTS

The authors highly appreciate the valuable technical assistance of Mrs. E. Asztalos-Turóczy and the financial help by OTKA CO182/A, FEFA II/241, and OTKA T017570.

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SEPARATION AND IDENTIFICATION OF TOFISOPAM STEREOISOMERS BY HYPHENATED HPLC-CD TECHNIQUE

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SEPARATION AND IDENTIFICATION OF TOFISOPAM STEREOISOMERS BY HYPHENATED HPLC-CD TECHNIQUE

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ACKNOWLEDGMENTS

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