![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A rapid and sensitive Reversed-Phase High Pressure Liquid Chromatographic (RP-HPLC) method has been developed for the quantitative determination of nitrendipine in human plasma.
A Hypersil C18 150×4.6 mm, 5 μm analytical column was used with a mixture of CH3CN-H2O at a volume ratio 50:50 v/v. Detection was performed with a variable wavelength UV-VIS detector at 238 nm. The detection limit was 0.4 ng/mL.
Nimodipine was used as internal standard (IS) at a concentration of 12 ng/mL.
The statistical evaluation of the method was examined performing intra-day and inter-day calibration.
Nitrendipine was extracted from human plasma matrices by Solid Phase Extraction (SPE) technique instead of conventional liquid-liquid extraction methods. Recovery of nitrendipine in spiked samples was 97.75±5.43.
The method was applied to a preliminary pharmacokinetic study in which an oral dose of 20 mg of nitrendipine was given to eight healthy volunteers.