Abstract
A Reversed-Phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous and separate determination of cis and trans-permethrin and piperonyl butoxide in a shampoo formulation is described and fully validated. This method entails the quantification of both components by external standard and ultraviolet detection at two wavelengths (201 and 287nm). A C8 Lichrosorb column and a three component mobile phase, isocratically delivered was used.
Results for process validation parameters – selectivity, precision, linearity, and recovery - were in agreement with international specifications. This is a simple method that requires no sample clean up prior to its injection in the system.