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Abstract
Human serum albumin was purified by size-exclusion liquid chromatography. The glycated albumin in the effluent was selectively measured by a post-column reaction detector. The selective reaction mechanism is to measure the amount of Amadori form of glycated albumin. According to the Maillard reaction, glycosylated amines are rearranged to Amadori form ketoamines which can produce superoxide in strong alkaline solution, and this superoxide produces chemiluminescence with lucigenin. The chemiluminescence intensity was related to the concentration of glycated albumin in albumin while the fluorescence intensity was the same.