pH-MODULATED STEPWISE ELUTION CCC AND ITS APPLICATION TO THE PREPARATIVE SEPARATION OF HYDROXYANTHRAQUINONE COMPOUNDS FROM TRADITIONAL CHINESE MEDICINAL HERBS

Fuquan Yang Beijing Research Center for Separation, & Purification Technology of Natural Products, Beijing Institute of New Technology Application, Beijing, 100035, P.R. China

Tianyou Zhang Beijing Research Center for Separation, & Purification Technology of Natural Products, Beijing Institute of New Technology Application, Beijing, 100035, P.R. China

Guoqin Xu Beijing Research Center for Separation, & Purification Technology of Natural Products, Beijing Institute of New Technology Application, Beijing, 100035, P.R. China

F. Edward Chou

Yoichiro Ito Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, U.S.A.

Abstract

Analytical and preparative high-speed countercurrent chromatography was successfully used for the separation of hydroxyanthraquinones from traditional Chinese medicine such as Rheum officinale Baill (Dahuang) and Polygonum cuspidatum Sieb. Et Zucc (Huzhang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion, and aloe-emodin were isolated each at over 98% purity.

ACKNOWLEDGMENTS

Financial support from Beijing Academy of Science & Technology and Beijing Commission of Science & Technology is gratefully acknowledged. We also thank senior engineer Xining Li for the excellent technical assistance with the research work.

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pH-MODULATED STEPWISE ELUTION CCC AND ITS APPLICATION TO THE PREPARATIVE SEPARATION OF HYDROXYANTHRAQUINONE COMPOUNDS FROM TRADITIONAL CHINESE MEDICINAL HERBS

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