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Journal of Liquid Chromatography & Related Technologies Volume 24, 2001 - Issue 11-12
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Original Articles

COUNTERCURRENT CHROMATOGRAPHY IN THE PREPARATIVE SEPARATION OF PLANT-DERIVED NATURAL PRODUCTS

K. Hostettmann Institut de Pharmacognosie et Phytochimie, Université de Lausanne, Lausanne, CH-1015, SwitzerlandCorrespondence[email protected]
&
A. Marston Institut de Pharmacognosie et Phytochimie, Université de Lausanne, Lausanne, CH-1015, Switzerland
Pages 1711-1721 | Received 29 Aug 2000, Accepted 14 Nov 2000, Published online: 06 Feb 2007
 
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Abstract

The versatility of high-speed countercurrent chromatography (HSCCC) makes it an ideal method for the separation of plantderived natural products. The technique can handle complex polar or apolar crude plant extracts equally well as purified fractions. Sample quantities can vary from several milligrams to tens of grams on the same instrument. Difficult isolation problems, with very similar compounds, can be attempted. Different applications that explore these possibilities are described here.

ACKNOWLEDGMENT

The Swiss National Science Foundation is gratefully acknowledged for financial support.

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