![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A simple, accurate, and rapid capillary micellar electrokinetic chromatography method was developed for the determination of gastrodin in rat blood. All experiments were performed in a 47 cm (40 cm effective length) × 75 μm i.d. uncoated fused-silica capillary with UV detection at 200 nm. A running buffer composed of 50 mM sodium tetraborate, 15 mM sodium dodecyl sulfate (SDS) (pH=9.50) was found to be suitable. The method was linear in the range of 2.50∼200.0 μg/mL (R=0.999), and the detection limit was 0.50 μg/mL. Only a small amount of blood (about 50 μL) was required for each analysis.
The recovery of gastrodin in rat blood was 91.51∼96.39%. This method was suitable for the determination of gastrodin after femoral vein injection and could be used for pharmaco-kinetic studies of this drug in rat blood.
ACKNOWLEDGMENTS
This work was supported by the National Natural Science Foundation of China (No. 29875012, 30070209) and Teacher Foundation of Chinese Education Ministry. We also thank Dr. Han Dong and Zuo Feng for their kindly help in calculating the pharmacokinetic parameters of this work.