Abstract
High-speed countercurrent chromatography was used for the prefractionation of flavonoids from the crude extracts of Pericarpium citri reticulatae, Radix puerariae, Radix glycyrrhizae, Radix scutellariae, and Flos genkwa. All separations were performed only with a two-phase system composed of CHCl3 : CH3OH :H2O=4 : x : 2. The x was changed for each sample between 2.5∼4.5. The fractionated components were identified by thin-layer chromatography, which confirmed this solvent system was versatile and very useful for prefractionation of flavonoids.
ACKNOWLEDGMENTS
This work was supported by the National Natural Science Foundation and the Yunnan Province's, Natural Science Foundation of China.