IMMUNOAFFINITY COLUMN FOR ISOLATION OF BIOACTIVE COMPOUNDS USING MONOCLONAL ANTIBODIES

ABSTRACT

Anti-solamargine and anti-ginsenoside Rb1 monoclonal antibodies were used for preparation of an immunoaffinity column. Total solasodine glycosides were separated directly from the crude extract of Solanum khasianum fruit, by the established immunoaffinity column. This method was specific for solasodine glycosides, which was detected by thin layer chromatography and eastern blotting. An immunoaffinity column, using anti-ginsenoside Rb1 monoclonal antibody, has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng).

Keywords:

• Immunoaffinity column
• Monoclonal antibodies
• ELISA

Acknowledgments