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Abstract
This work describes the development of HPLC and micellar electrokinetic capillary chromatography (MEKC) methods, towards the selectivity for biotin analysis and its use for the analysis of pharmaceutical preparation (gel as a filling of a capsule). The isolation of biotin from this matrix was possible after dissolution of the gel from the capsules in hexane, and extraction of fat‐soluble vitamins. The separation of the biotin extract by HPLC did not give good results. Therefore, separation of biotin from interfering substances was performed with high resolution by MEKC with diode‐array detector (DAD). The optimization of composition of the buffer in MEKC was obtained for six different concentration of sodium dodecyl sulfate (SDS). The relative standard deviation (RSD) for migration time was 0.91% and for peak area the RSD was better than 2.5%. The detection limit ranged from 5 to 200 µg/mL. The limit of detection level for biotin was 0.15 µg/mL.