Preparation of Strong Cation Exchange Packings Based on Monodisperse Poly(Glycidyl Methacrylate‐co‐Ethylenedimethacrylate) Particles and Their Application

Abstract

Monodisperse macroporous poly(glycidyl methacrylate‐co‐ethylenedimethacrylate) (P_GMA/EDMA) particles were synthesized by a single‐step swelling and polymerization method. Based on this medium, a strong cation ion exchange resin was prepared by a new chemically modified method. It is demonstrated that the prepared resin in this study has advantages for biopolymer separation, high column efficiency, low column back pressure, and good resolution for proteins. The bioactivity recovery for lysozyme (Lys) with the resin was measured to be 102 ± 5%. The resin was also used for the separation and purification of recombinant human interferon‐γ (rhIFN‐γ) to raise its purity, in the coarse extract containing 0.2 mol/L guanidine hydrochloride, from 36% to 93%.

Keywords:

• Monodisperse poly(glycidyl methacrylate‐co‐ethylenedimethacrylate) particles
• Strong cation exchange chromatography
• Resin
• Protein separation
• Interferon‐γ

Acknowledgments

Financial support for this research was provided by the National Scientific Foundation in China (Nos. 39880003 and 20175016 ) and they are gratefully acknowledged.