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Abstract
An HPLC procedure for the simultaneous determination of metformin in combination with glipizide and gliclazide in pharmaceutical preparations is described. A Zorbax XDB C18 15 cm analytical column and UV detection at wavelength 226 nm were used. In this study, various mobile phase variables were studied to determine the effects that each had on metformin, glipizide, and gliclazide. The reproducibilities in combination I for metformin and glipizide were 1.39%, 1.30%, and in combination II for metformin and gliclazide were 1.28%, 1.75%, respectively. The determinations of glipizide, metformin (combination I), and gliclazide, metformin (combination II) gave recoveries with respect to the values declared by the manufacturers for glipizide and metformin (combination‐I) in the range 96.1–103.4%, 95.0–100.0% using peak areas, and 96.4–103.6%, 97.3–102.5% using peak heights, respectively. The recoveries for the gliclazide and metformin (combination II) were in the range 94.7–100.8%, 96.2–101.2% using peak areas, and 94.3–101.5%, 97.8–102.5% using peak heights, respectively.
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Acknowledgments
The authors are thankful to the Head, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, and Wockhardt Research Centre for providing the facility for this research work.